Gastric cancer (GC) accounts for a main cause of cancer-related deaths.This study sought DULSE RICE CAKES UNSALTED for molecular mechanism of miR-490-5p/DTL axis in affecting GC progression, thus bringing new hope for treatment of GC.Expression data of differentially expressed miRNAs and mRNAs in GC tissue from TCGA database were analyzed.MiR-490-5p and DTL mRNA expression levels in GC were evaluated with qRT-PCR.
Cell viability was confirmed with CCK-8 method.Cell cycle distribution and apoptosis were analyzed with flow cytometry.Cell migratory and invasive potential was proved with Transwell assay.The targeted relationship between DTL and miR-490-5p was analyzed with dual-luciferase assay.
The results indicated a decreased miR-490-5p level in GC cells.MiR-490-5p upregulation hampered proliferation, migration, invasion and promote cell apoptosis.DTL was the target of and inversely associated with miR-490-5p, and it could remarkably induce the carcinogenesis of GC.MiR-490-5p mediated GC 1036 cell progression by DTL repression.
In conclusion, miR-490-5p and DTL may be valuable in diagnosis and treatment for GC.